Plasmid_Backbone
pBBknock

Part:BBa_J107077:Design

Designed by: Michela Casanova, Lorenzo Pasotti, Susanna Zucca, Nicolo Politi, Ilaria Massaiu, Cinzia Calvio, Gabriella Cusella, Paolo Magni   Group: Magni Lab   (2016-01-02)


pBBknock standard vector for allelic replacement


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3865
    Illegal NheI site found at 28
    Illegal NheI site found at 51
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2521
    Illegal NotI site found at 3871
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3865
    Illegal BglII site found at 1023
    Illegal XhoI site found at 3090
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3865
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3865
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3880
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 401
    Illegal NgoMIV site found at 573
    Illegal AgeI site found at 924
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1070


Design Notes

See BBa_J107112.


Source

Synthetic DNA obtained via GenScript synthesis service.

References